Zarodniki Fusarium arthrosporioides

[Jakusz]

Gdyby ktoś chciał konkretną metodę pozyskania zarodników (zapewne w dużym stężeniu, bo jak wynika z opisu, odpłukiwanie następowało z całej grzybni) - znalazłem to:

Fusarium culture
All Fusarium strains were stored as conidial suspensions
in 30% glycerol at −80°C, regularly transferred
to a growth medium and cultivated at 24°C in darkness.
The two pathogens, F. oxysporum f.sp. lycopersici strain
Fol 007 (provided by B.J. Cornelissen, University
396 Eur J Plant Pathol (2008) 122:395–401
of Amsterdam, The Netherlands) and F. oxysporum
f.sp. radicis-lycopersici strain Forl 101587 (purchased
from Centraalbureau voor Schimmelcultures,
The Netherlands), the biocontrol strain F. oxysporum
strain Fo 47 (provided by C. Steinberg, INRA/
Université de Bourgogne, Dijon, France) and the
unspecific non-pathogenic F. oxysporum strain Fo
135 (provided by M. Lemmens, IFA Tulln, Austria)
were cultivated on Czapek Dox medium (Duchefa
Biochemie, Haarlem, The Netherlands). Under sterile
conditions, fungal culture plates (2 to 3 weeks
old) were flooded with sterile distilled water (SDW)
and the suspension obtained was filtered through
three layers of filter paper (fleece filters, 20–150 μm
pore diam Laporte Ges.m.b.H., Wels, Austria) to
separate the microconidia from the mycelium. The
conidial concentration was determined using a
haemocytometer and adjusted to a final concentration
of 1×107 microconidia ml−1.