Orzęski

[Pickul]

francisco beautiful specimen and good video

[francisco]

Thanks

[francisco]

Hello
Ciliate very elastic: Amphileptus
Francisco

[JohnyM]

Misiek, Francisco : If youre using phase plates for normal objectives, you can get a little worse darkfield [DF] (low light because of outer aperture ring) or technique called circular obligue light [COL]. On above videos is just regular darkfield. If you look through auxilary microscope (for centering phase plates) and inner disc of plate is bigger than objective aperture youll get DF. If its smaller youll get COL.
Edit: Theres a nice short article about COL and some photos:

Kod: Zaznacz cały

microscopy-uk.org.uk/mag/artdec02/pjcol.html

Edit2: You can also say Its a poor mans phase contrast. You still get interference effect (and halos). Using a objective with phase plate will just simply increase the phase shift.

[kriscavok]

Our little forum is getting worldwide

[planapo]

quality matters (:

[francisco]

Thanks for the interesting information
I use phase contrast objective.
For lighting oblique phase condenser put in position 1. 20X objective
Francisco

[marcin93]

Orzęsek ob. Nikon E Plan 40/0.65
Przed i po rozleceniu się.
Dosyć trudne jest to ciemne pole do focenia. Ja swoim telefonem już raczej więcej nie wyciągnę

[marcin93]

Całkiem duży orzęsek . Nikon E Plan 40/0.65

[marcin93]

Filmik z wyżej pokazanym orzęskiem i eugleną.

[francisco]

Trichodina, parasite ciliate

Francisco

[sebba28]

Beautiful video,Francisco.
Did you used cctv camera or digital camera?

[francisco]

Thanks sebba28
I used the camera cctv
Francisco